Zap70 Mouse shRNA Plasmid (Locus ID 22637)
CAT#: TL509808
Zap70 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 7,740.00
货期*
2周
规格
Cited in 1 publication. |
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Specifications
Product Data | |
Product Name | Zap70 Mouse shRNA Plasmid (Locus ID 22637) |
Locus ID | 22637 |
UniProt ID | P43404 |
Synonyms | mrtle; mur; S; Srk; ZAP-; ZAP-70 |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | Zap70 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22637). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | BC029727, NM_001289612, NM_001289765, NM_001289766, NM_009539, NM_009539.1, NM_009539.2, NM_009539.3, NM_001289612.1, NM_001289765.1, NM_001289766.1 |
Summary | This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
---|
Morpholino-based correction of hypomorphic ZAP70 mutation in an adult with combined immunodeficiency
,null,
The Journal of Allergy and Clinical Immunology
,PubMed ID 28216435
[Zap70]
|
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