eNOS (NOS3) Human shRNA Plasmid Kit (Locus ID 4846)

CAT#: TL320432

NOS3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 5,740.00


货期*
现货

规格
    • 1 kit

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Specifications

Product Data
Product Name eNOS (NOS3) Human shRNA Plasmid Kit (Locus ID 4846)
Locus ID 4846
UniProt ID P29474
Synonyms ECNOS; eNOS
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components NOS3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 4846). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_000603, NM_001160109, NM_001160110, NM_001160111, NM_000603.1, NM_000603.2, NM_000603.3, NM_000603.4, NM_001160109.1, NM_001160110.1, NM_001160111.1, BC063294, BC063294.1, BC069465, NM_000603.5, NM_001160109.2
Summary Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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