GRB10 Human shRNA Plasmid Kit (Locus ID 2887)

Specifications

Product Data
Product Name	GRB10 Human shRNA Plasmid Kit (Locus ID 2887)
Locus ID	2887
UniProt ID	Q13322
Synonyms	Grb-10; GRB-IR; IRBP; MEG1; RSS
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	GRB10 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2887). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001001549, NM_001001550, NM_001001555, NM_005311, NM_001350814, NM_001350815, NM_001350816, NM_001001555.1, NM_001001555.2, NM_005311.1, NM_005311.2, NM_005311.3, NM_005311.4, NM_001001549.1, NM_001001549.2, NM_001001550.1, NM_001001550.2, BC024285, BC024285.1, NM_001001555.3, NM_001001550.3
Summary	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.