Glycophorin A (GYPA) Human shRNA Plasmid Kit (Locus ID 2993)

Specifications

Product Data
Product Name	Glycophorin A (GYPA) Human shRNA Plasmid Kit (Locus ID 2993)
Locus ID	2993
UniProt ID	P02724
Synonyms	CD235a; GPA; GPErik; GPSAT; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	GYPA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2993). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001308187, NM_001308190, NM_002099, NM_002099.1, NM_002099.2, NM_002099.3, NM_002099.4, NM_002099.5, NM_002099.6, NM_002099.7, BC005319, BC005319.1, BC013328, NM_002099.8
Summary	Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.