Phospholipase A2 IIA (PLA2G2A) Human shRNA Plasmid Kit (Locus ID 5320)

Specifications

Product Data
Product Name	Phospholipase A2 IIA (PLA2G2A) Human shRNA Plasmid Kit (Locus ID 5320)
Locus ID	5320
UniProt ID	P14555
Synonyms	MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	PLA2G2A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5320). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000300, NM_001161727, NM_001161728, NM_001161729, NM_000300.1, NM_000300.2, NM_000300.3, NM_001161727.1, NM_001161728.1, NM_001161729.1, BC005919, BM708748, NM_000300.4, NM_001161727.2, NM_001161728.2
Summary	The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.