SLC7A7 Human shRNA Plasmid Kit (Locus ID 9056)

Specifications

Product Data
Product Name	SLC7A7 Human shRNA Plasmid Kit (Locus ID 9056)
Locus ID	9056
UniProt ID	Q9UM01
Synonyms	LAT3; LPI; MOP-2; y+LAT-1; Y+LAT1
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	SLC7A7 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 9056). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001126105, NM_001126106, NM_003982, NR_040448, NM_003982.1, NM_003982.2, NM_003982.3, NM_001126105.1, NM_001126105.2, NM_001126106.1, NM_001126106.2, BC003062, BC003062.2, BC010107, NM_001126106.4, NM_001126105.3, NM_003982.4
Summary	The protein encoded by this gene is the light subunit of a cationic amino acid transporter. This sodium-independent transporter is formed when the light subunit encoded by this gene dimerizes with the heavy subunit transporter protein SLC3A2. This transporter is found in epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in this gene are a cause of lysinuric protein intolerance (LPI). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.