SP100 Human shRNA Plasmid Kit (Locus ID 6672)

Specifications

Product Data
Product Name	SP100 Human shRNA Plasmid Kit (Locus ID 6672)
Locus ID	6672
UniProt ID	P23497
Synonyms	lysp100b
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	SP100 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6672). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001080391, NM_001206701, NM_001206702, NM_001206703, NM_001206704, NM_003113, NM_001080391.1, NM_003113.1, NM_003113.2, NM_003113.3, NM_001206704.1, NM_001206703.1, NM_001206702.1, NM_001206701.1, BC011562, NM_001206701.2, NM_001080391.2, NM_003113.4
Summary	This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.