TRAK1 Human shRNA Plasmid Kit (Locus ID 22906)

Specifications

Product Data
Product Name	TRAK1 Human shRNA Plasmid Kit (Locus ID 22906)
Locus ID	22906
UniProt ID	Q9UPV9
Synonyms	EIEE68; MILT1; OIP106
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	TRAK1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22906). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC015922, NM_001042646, NM_001265608, NM_001265609, NM_001265610, NM_014965, NM_001349245, NM_001349246, NM_001349247, NM_001349248, NM_001349249, NR_146089, NM_014965.1, NM_014965.2, NM_014965.3, NM_014965.4, NM_001042646.1, NM_001042646.2, NM_001265610.1, NM_001265609.1, NM_001265608.1, BC015922.1, NM_001265608.2, NM_001042646.3, NM_014965.5
Summary	Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.