UBE3A Human shRNA Plasmid Kit (Locus ID 7337)

Specifications

Product Data
Product Name	UBE3A Human shRNA Plasmid Kit (Locus ID 7337)
Locus ID	7337
UniProt ID	Q05086
Synonyms	ANCR; AS; E6-AP; EPVE6AP; HPVE6A
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	UBE3A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 7337). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000462, NM_130838, NM_130839, NM_001354505, NM_001354506, NM_001354507, NM_001354508, NM_001354509, NM_001354511, NM_001354512, NM_001354513, NM_001354523, NM_001354526, NM_001354538, NM_001354539, NM_001354540, NM_001354541, NM_001354542, NM_001354543, NM_001354544, NM_001354545, NM_001354546, NM_001354547, NM_001354548, NM_001354549, NM_001354550, NM_001354551, NR_148916, NM_130838.1, NM_130839.1, NM_130839.2, NM_000462.1, NM_000462.2, NM_000462.3, BC002582, BC002582.2, BC009271, NM_130839.5, NM_130838.4, NM_000462.5
Summary	This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.