GPR172A (SLC52A2) Human shRNA Plasmid Kit (Locus ID 79581)

Specifications

Product Data
Product Name	GPR172A (SLC52A2) Human shRNA Plasmid Kit (Locus ID 79581)
Locus ID	79581
UniProt ID	Q9HAB3
Synonyms	BVVLS2; D15Ertd747e; GPCR41; GPR172A; hRFT3; PAR1; RFT3; RFVT2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	SLC52A2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 79581). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001253815, NM_001253816, NM_024531, NR_045600, NM_024531.1, NM_024531.2, NM_024531.3, NM_024531.4, NM_001253815.1, NM_001253816.1, BC002917, NM_001363118, NM_001363121, NM_001363120, NM_001363122, NM_001253815.2, NM_001253816.2, NM_024531.5
Summary	This gene encodes a membrane protein which belongs to the riboflavin transporter family. In humans, riboflavin must be obtained by intestinal absorption because it cannot be synthesized by the body. The water-soluble vitamin riboflavin is processed to the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) which then act as intermediaries in many cellular metabolic reactions. Paralogous members of the riboflavin transporter gene family are located on chromosomes 17 and 20. Unlike other members of this family, this gene has higher expression in brain tissue than small intestine. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in this gene have been associated with Brown-Vialetto-Van Laere syndrome 2 - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia. [provided by RefSeq, Jul 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.