SHC (SHC1) Human shRNA Plasmid Kit (Locus ID 6464)

Specifications

Product Data
Product Name	SHC (SHC1) Human shRNA Plasmid Kit (Locus ID 6464)
Locus ID	6464
UniProt ID	P29353
Synonyms	SHC; SHCA
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	SHC1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6464). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001130040, NM_001130041, NM_001202859, NM_003029, NM_183001, NM_003029.1, NM_003029.2, NM_003029.3, NM_003029.4, NM_183001.1, NM_183001.2, NM_183001.3, NM_183001.4, NM_001130041.1, NM_001130040.1, NM_001202859.1, BC014158, BC014158.2, BC011813, BC017283, BC033925, NM_001130041.2, NM_001130040.2, NM_003029.5, NM_183001.5, NM_001202859.2
Summary	This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.