E2f1 Mouse shRNA Plasmid (Locus ID 13555)

CAT#: TG509487

E2f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided



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CNY 4,790.00


货期*
现货

规格
    • 1 kit

Cited in 1 publication.

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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Specifications

Product Data
Product Name E2f1 Mouse shRNA Plasmid (Locus ID 13555)
Locus ID 13555
UniProt ID Q61501
Synonyms E2F-1; mKIAA4009; Tg(Wnt1-cre)2Sor
Vector pGFP-V-RS
Format Retroviral plasmids
Kit Components E2f1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 13555). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq BC052160, NM_001291105, NM_007891, NM_007891.1, NM_007891.2, NM_007891.3, NM_007891.4, NM_007891.5, NM_001291105.1
Summary Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:11672531, PubMed:20176812). Positively regulates transcription of RRP1B (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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