MAP4K4 Human shRNA Plasmid Kit (Locus ID 9448)

Specifications

Product Data
Product Name	MAP4K4 Human shRNA Plasmid Kit (Locus ID 9448)
Locus ID	9448
UniProt ID	O95819
Synonyms	FLH21957; HEL-S-31; HGK; MEKKK4; NIK
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	MAP4K4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 9448). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_001242559, NM_001242560, NM_004834, NM_145686, NM_145687, NM_145686.1, NM_145686.2, NM_145686.3, NM_004834.1, NM_004834.2, NM_004834.3, NM_004834.4, NM_145687.1, NM_145687.2, NM_145687.3, NM_001242560.1, NM_001242559.1, BC010909, BC040077, BC045694, BC051791, BC054038, BC064691, BC065036, BC156089, BC156887, NM_001242559.2, NM_004834.5, NM_001242560.2, NM_145687.4
Summary	The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.