MADH7 (SMAD7) Human shRNA Plasmid Kit (Locus ID 4092)
CAT#: TG320409
SMAD7 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | MADH7 (SMAD7) Human shRNA Plasmid Kit (Locus ID 4092) |
Locus ID | 4092 |
UniProt ID | O15105 |
Synonyms | CRCS3; MADH7; MADH8 |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | SMAD7 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 4092). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | NM_001190821, NM_001190822, NM_001190823, NM_005904, NM_005904.1, NM_005904.2, NM_005904.3, NM_001190822.1, NM_001190821.1, NM_001190823.1, BC074818, BC074818.2, BC074819 |
Summary | The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition
,Parikh, A;Lee, C;Joseph, P;Marchini, S;Baccarini, A;Kolev, V;Romualdi, C;Fruscio, R;Shah, H;Wang, F;Mullokandov, G;Fishman, D;D'Incalci, M;Rahaman, J;Kalir, T;Redline, RW;Brown, BD;Narla, G;Difeo, A;,
Nat Commun, Jan 2014.
,PubMed ID 24394555
[Smad7]
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