Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) Human shRNA Plasmid Kit (Locus ID 3295)

Specifications

Product Data
Product Name	Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) Human shRNA Plasmid Kit (Locus ID 3295)
Locus ID	3295
UniProt ID	P51659
Synonyms	DBP; MFE-2; MFP-2; MPF-2; PRLTS1; SDR8C1
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	HSD17B4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 3295). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_000414, NM_001199291, NM_001199292, NM_001292027, NM_001292028, NM_000414.1, NM_000414.2, NM_000414.3, NM_001199292.1, NM_001199291.1, NM_001199291.2, NM_001292028.1, NM_001292027.1, BC003098, NM_001199291.3, NM_001292027.2, NM_000414.4, NM_001292028.2, NM_001199292.2
Summary	The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.