Pparg Mouse shRNA Plasmid (Locus ID 19016)
CAT#: TF510219
Pparg - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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经常一起买 (1)
Specifications
Product Data | |
Product Name | Pparg Mouse shRNA Plasmid (Locus ID 19016) |
Locus ID | 19016 |
UniProt ID | P37238 |
Synonyms | Nr1; Nr1c3; PPA; PPAR; Ppar-; PPAR-gamma; PPAR-gamma2; PPARgamma; PPARgamma2 |
Vector | pRFP-C-RS |
Format | Retroviral plasmids |
Kit Components | Pparg - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 19016). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free. |
RefSeq | BC021798, NM_001127330, NM_011146, NM_011146.1, NM_011146.2, NM_011146.3, NM_001127330.1, NM_001127330.2 |
Summary | This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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Mapping Distinct Bone Marrow Niche Populations and Their Differentiation Paths
,null,
Cell reports
,PubMed ID 31291568
[Pparg]
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