TLK2 Human shRNA Plasmid Kit (Locus ID 11011)

Specifications

Product Data
Product Name	TLK2 Human shRNA Plasmid Kit (Locus ID 11011)
Locus ID	11011
UniProt ID	Q86UE8
Synonyms	HsHPK; PKU-ALPHA
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	TLK2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 11011). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001112707, NM_001284333, NM_001284363, NM_006852, NM_001330418, NM_006852.1, NM_006852.2, NM_006852.3, NM_001112707.1, NM_001284363.1, NM_001284333.1, BC013736, BC044925, BC060511, BM668399, NM_001284333.2, NM_006852.6
Summary	This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.