GGCT Human shRNA Plasmid Kit (Locus ID 79017)

Specifications

Product Data
Product Name	GGCT Human shRNA Plasmid Kit (Locus ID 79017)
Locus ID	79017
UniProt ID	O75223
Synonyms	C7orf24; CRF21; GCTG; GGC
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	GGCT - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 79017). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001199815, NM_001199816, NM_001199817, NM_024051, NR_037669, NM_024051.1, NM_024051.2, NM_024051.3, NM_001199817.1, NM_001199816.1, NM_001199815.1, BC000625, BC000625.2, BC005356, BC013937, BC019243, NM_024051.4, NM_001199817.2, NM_001199815.2
Summary	The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.