Caveolin 1 (CAV1) Human shRNA Plasmid Kit (Locus ID 857)

CAT#: TF314183

CAV1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided



Need custom shRNA service?
Get a free quote

CNY 4,790.00


货期*
现货

规格
    • 1 kit

Cited in 1 publication.

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


CAV1 mouse monoclonal antibody,clone OTI2C4
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Product Name Caveolin 1 (CAV1) Human shRNA Plasmid Kit (Locus ID 857)
Locus ID 857
UniProt ID Q03135
Synonyms BSCL3; CGL3; LCCNS; MSTP085; PPH3; VIP21
Vector pRFP-C-RS
Format Retroviral plasmids
Kit Components CAV1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 857). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001172895, NM_001172896, NM_001172897, NM_001753, NM_001753.1, NM_001753.2, NM_001753.3, NM_001753.4, NM_001172895.1, NM_001172896.1, NM_001172897.1, BC082246, BC082246.1, BC006432, BC009685, NM_001753.5, NM_001172897.2
Summary The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

Customer Reviews 
Loading...