MLH1 (NM_000249) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region full length of MLH1
Tag	N-GST and C-HIS
Predicted MW	84.6 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	50mM Tris, pH8.0, 8M Urea
Storage	Store at -80°C after receiving vials.
Stability	Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_000240
Locus ID	4292
UniProt ID	P40692
Refseq Size	2662
Cytogenetics	3p22.2
Refseq ORF	2268
Synonyms	COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1
Summary	The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
Protein Families	Druggable Genome
Protein Pathways	Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

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