Recombinant IL-34 (Interleukin-34)，Mouse

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MNENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 25.52 kDa. The protein migrates about 25 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <30 ng/mL. The specific activity of recombinant mouse IL-34 is > 3.3 x 10⁴ IU/mg.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q8R1R4
Synonyms	2010004A03Rik,Il34
Summary	Interleukin 34 (IL-34) is a 23.98 kDa cytokine with 216 amino acid residues. IL-34 is a ligand of the colony-stimulating factor 1 receptor (CSF-1R) and is mainly secreted from the adrenal, frontal lobe, and cortex. IL-34 play a vital role in the proliferation, survival, differentiation, and migration of myeloid cells. These functions are mediated by IL-33 activating ERK1 /2, JAK, and PI3K/AKT signal pathways.

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