Recombinant TGF beta 3 (Transforming growth factor beta 3)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 10⁷ IU/mg.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P10600
Synonyms	ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3
Summary	Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution.

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