Recombinant BMP-11 (Bone morphogenetic protein-11)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 13.40 kDa. The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <11 ng/mL. Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <4 ng/mL.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	O95390
Synonyms	Growth/Differentiation Factor-11, GDF-11
Summary	Bone Morphogenetic Protein-11 (BMP-11), known as Growth differentiation factor 11 (GDF11), is an extracellular multifunctional signaling cytokine that is also a member of the TGFβ family. BMP-11 can bind with the TGFβ receptor and is involved in SMAD protein signal transduction. Moreover, it promotes the formation of blood vessels and nerves that can control anterior-posterior patterning.

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