Wee2 (NM_201370) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR208795 protein sequence Red=Cloning site Green=Tags(s) MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKG SPGPDLWKSVSGPPKCPETPVLHSKSKPPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLVDE KTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFE IEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHV VIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDS PVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIA VAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDE LQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	62.2 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_958758
Locus ID	381759
UniProt ID	Q66JT0
Refseq Size	2886
Cytogenetics	6 B1
Refseq ORF	1668
Synonyms	Gm1065; Wee1B
Summary	Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.[UniProtKB/Swiss-Prot Function]

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