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Wee2 (NM_201370) Mouse Tagged ORF Clone
CAT＃: MR208795
- TrueORF®
Wee2 (Myc-DDK-tagged) - Mouse WEE1 homolog 2 (S. pombe) (Wee2)
ORF Plasmid: tGFP
"NM_201370" in other vectors (4)
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CNY 4,144.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm1065; Wee1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208795 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACACAGAGACTGACCAGGGACTGAACAAGAAATTAAGCTTCTCCTTTTGTGAAGAAGACACTG AGAGTGAAGGGCAGATGACTGCACAAGACATCGGAGGGGCCCAGAGCCAAAAGCCTGGGAGGGAAGAAAG TGAGGAGTTGGAGGGTCCCGTGCCTCCTACCAGAGATGAACTTCATACTTCTCTGAGCAGAGACAAGGGA AGTCCAGGTCCTGATCTTTGGAAATCAGTTTCAGGCCCTCCCAAATGCCCAGAGACACCAGTATTACACA GCAAGAGCAAGCCGCCAGTCCCTACCAACTTCTCCACTCCCAAAAATTCGCTGGGTCAGCCAGAGATTTC TCTGCTAGAGAAGCTCTCCTCCCGAAGCTCTAGACATATGAGGCTCACACCTGCTTCCCTTGTGGATGAG AAGACCTCTTTGTCTCTGGTCAATATCAACCCATTCACTCCGGAGACCTACAGAAAATTACTCCTTCAAT CAAAGGGCAAGAGGAAGACCAGAGACTATCTTGAGGAAACTGGAGAAGAGGAAAGCAAATCAGTACAGTG GCTGCCTGCTAAGAGAAGTATTCTACAAGAAACTAATATGGCTTCCCGCTATGAAAAAGAATTCTTCGAG ATAGAAAAAATCGGGGTTGGAGAATTTGGGACAGTCTACAAGTGCATTAAGAGGCTGGATGGATGCATTT ATGCAATAAAGCGCTCTGCAAAGTCCTTTTCAGGATTATCAAATGAATTGGATTTGCATGAAGTTTATGC TCATGCAGTGCTTGGCCATCATCCCCATGTGGTACGCTACTACTCATCATGGATAGAAGATGATCATGTG GTCATTCAAAATGAATACTGTAATGGTGGGAGCCTGCAAGCTGCTATATCTGAAAACACTGCGTCCAATA ATCACTTCCAAGAGCCCAAACTCAAAGACATCCTTCTGCAGATTTCCTTGGGACTTAAGTACATCCACAA TTCTGGCATGGTACACCTGGACATCAAACCCAGTAACATCTTCATTTGTCATAAGATGCAGTGTGACTCT CCTGTAGGCCCAGAAGAGGCTGAAAGTGAAGCTGACTGGTTTCTCAATGCCAGTGTGATGTATAAAATTG GTGACCTGGGCCACGCGACATCAATCAGCAAACCTAAAGTGGAAGAAGGAGATACTCGTTTCCTGGCTAA TGAGATCTTGCAAGAGAATTACCAACACCTCCCCAAAGCAGACATATTTGCCTTAGGATTAACAATCGCA GTGGCTGCAGGAGCAGAGTCTTTACCCATCAATGGTGACATGTGGCATCATATCCGCAAAGGGAACTTTC CAGAGATTTCCCAAGAGCTCTCGGATGACTTTTATGGTCTACTCAAGAACATGATCCACCCAGCTCCAAA GGAGAGACCATCTGCAGCAGCTCTGGCCAGAAGTCGAATTCTCTGGCCTTTCTTGGAGAAGACTGATGAA CTCCAGAAACAACTGAATTTGGAGAAATCCAAGACAGCCACACTGAAAAGGGAACTGAAAAAAGCTCGGC ATATCCAGACCCCTCAGAGAGAAGTCCACCATTGTTATTATCAGATCTGCAAAGGCTCAAGACACCTCCT GGTGGGAGGAAGGAGGAAGGCGCCCTCAAGCTTCACCCGTGGGACATCCTCCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208795 protein sequence
Red=Cloning site Green=Tags(s) MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKG SPGPDLWKSVSGPPKCPETPVLHSKSKPPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLVDE KTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFE IEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHV VIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDS PVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIA VAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDE LQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201370 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201370.1, NP_958758.1 |
| RefSeq Size | 2886 bp |
| RefSeq ORF | 1668 bp |
| Locus ID | 381759 |
| UniProt ID | Q66JT0 |
| MW | 62.2 kDa |
| Gene Summary | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201865 | Wee2 (untagged) - Mouse WEE1 homolog 2 (S. pombe) (Wee2), (10ug) |
CNY 4,144.00 |
|
| MG208795 | Wee2 (tGFP-tagged) - Mouse cDNA sequence BC052883 (BC052883) |
CNY 5,040.00 |
|
| MR208795L3 | Lenti ORF clone of Wee2 (Myc-DDK-tagged) - Mouse WEE1 homolog 2 (S. pombe) (Wee2) |
CNY 6,460.00 |
|
| MR208795L4 | Lenti ORF clone of Wee2 (mGFP-tagged) - Mouse WEE1 homolog 2 (S. pombe) (Wee2) |
CNY 6,460.00 |
