PCNA (NM_002592) Human Recombinant Protein
CAT#: TP301741
Recombinant protein of human proliferating cell nuclear antigen (PCNA), transcript variant 1, 20 µg
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>RC201741 protein sequence
Red=Cloning site Green=Tags(s) MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGV NLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMP SGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALR YLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Predicted MW | 28.6 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_002583 |
Locus ID | 5111 |
UniProt ID | P12004 |
Refseq Size | 1355 |
Cytogenetics | 20p12.3 |
Refseq ORF | 783 |
Synonyms | ATLD2 |
Summary | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair |
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Other Versions
SKU | Description | Size | Price |
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LC400929 | PCNA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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LC405433 | PCNA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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LC430596 | PCNA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY400929 | Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 1 |
CNY 3,080.00 |
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LY405433 | Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 2 |
CNY 3,080.00 |
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LY430596 | Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 2 |
CNY 3,080.00 |
|
PH301741 | PCNA MS Standard C13 and N15-labeled recombinant protein (NP_002583) |
CNY 19,520.00 |
|
TP750150 | Purified recombinant protein of Human proliferating cell nuclear antigen (PCNA), transcript variant 2, full length, tag free, expressed in E.Coli, 50 ug |
CNY 2,040.00 |
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TP760481 | Purified recombinant protein of Human proliferating cell nuclear antigen (PCNA), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug |
CNY 2,040.00 |