GATA3 (NM_002051) Human Recombinant Protein
CAT#: TP301618
Recombinant protein of human GATA binding protein 3 (GATA3), transcript variant 2, 20 µg
View other "GATA3" proteins (8)
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>RC201618 protein sequence
Red=Cloning site Green=Tags(s) MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRA TVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSS LSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGG ASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTEGRECVNCGATSTPLWRRDGT GHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHN INRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTP TPMHPPSSLSFGPHHPSSMVTAMG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Predicted MW | 47.7 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Bioactivity | GATA3 Activity Verified in a DNA-binding Assay: DNA binding activity of GATA3 as a function of protein concentration. Sensitivity to competitor oligonucleotide demonstrates specificity of protein-DNA binding. EMSA assay (PMID: 26216189) |
Reference Data | |
RefSeq | NP_002042 |
Locus ID | 2625 |
UniProt ID | P23771 |
Refseq Size | 3067 |
Cytogenetics | 10p14 |
Refseq ORF | 1332 |
Synonyms | HDR; HDRS |
Summary | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009] |
Protein Families | Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
Citations (2)
The use of this Proteins has been cited in the following citations: |
---|
Gata3-controlled nucleosome eviction drives Myc enhancer activity in T-cell development and leukemia
,Belver, L;Yang, AY;Albero, R;Herranz, D;Brundu, FG;Quinn, SA;Perez-Duran, P;Alvarez, S;Gianni, F;Rashkovan, M;Gurung, D;Rocha, PP;Raviram, R;Reglero, C;Cortes, JR;Cooke, AJ;Wendorff, AA;Cordo, V;Meijerink, JP;Rabadan, R;Ferrando, AA;,
Cancer Discov
,PubMed ID 31519704
[GATA3]
|
Transactivation of human osteoprotegerin promoter by GATA-3
,Kao, SY;Stankovic, KM;,
Sci Rep
,PubMed ID 26216189
[GATA3]
|
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