NCF1 (NM_000265) Human Recombinant Protein
CAT#: TP301233
Recombinant protein of human neutrophil cytosolic factor 1 (NCF1), 20 µg
View other "NCF1" proteins (4)
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CNY 2,900.00
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Cited in 1 publication. |
CNY 600.00
Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>RC201233 representing NM_000265
Red=Cloning site Green=Tags(s) MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENR IIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYL MPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFL EPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYL QKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPL EEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Predicted MW | 44.5 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Bioactivity | Enzyme substrate (PMID: 29195919) |
Reference Data | |
RefSeq | NP_000256 |
Locus ID | 653361 |
UniProt ID | P14598 |
Refseq Size | 1409 |
Cytogenetics | 7q11.23 |
Refseq ORF | 1170 |
Synonyms | CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A |
Summary | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
Protein Pathways | Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils
,Nagarkoti, S;Dubey, M;Awasthi, D;Kumar, V;Chandra, T;Kumar, S;Dikshit, M;,
Biochim. Biophys. Acta
,PubMed ID 29195919
[NCF1]
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Other Versions
SKU | Description | Size | Price |
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LC400103 | NCF1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY400103 | Transient overexpression lysate of neutrophil cytosolic factor 1 (NCF1) |
CNY 3,080.00 |
|
PH301233 | NCF1 MS Standard C13 and N15-labeled recombinant protein (NP_000256) |
CNY 19,520.00 |
|
TP761217 | Purified recombinant protein of Human neutrophil cytosolic factor 1 (NCF1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug |
CNY 2,040.00 |