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CD44 (NM_001001391) Human Over-expression Lysate
CAT＃: LC424334
CD44 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "CD44" proteins (10)
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CNY 900.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Description | CD44 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221820 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 39.42 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001001391 |
| Locus ID | 960 |
| Cytogenetics | 11p13 |
| Protein Families | WB |
| Protein Pathways | ECM-receptor interaction, Hematopoietic cell lineage |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
|
MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells
,null,
Cancers
,PubMed ID 34067416
[CD44]
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Other Versions
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| LC400203 | CD44 HEK293T cell transient overexpression lysate (as WB positive control) |
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| LC424332 | CD44 HEK293T cell transient overexpression lysate (as WB positive control) |
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| LY400203 | Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 1 |
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| LY424332 | Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 2 |
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| LY424334 | Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 4 |
CNY 3,080.00 |
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| PH302455 | CD44 MS Standard C13 and N15-labeled recombinant protein (NP_001001389) |
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| PH321771 | CD44 MS Standard C13 and N15-labeled recombinant protein (NP_000601) |
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| TP321771 | Recombinant protein of human CD44 molecule (Indian blood group) (CD44), transcript variant 1, 20 µg |
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| TP720465 | Recombinant protein of human CD44 molecule (Indian blood group) (CD44), transcript variant 1 |
CNY 3,140.00 |
