SUPT5H (NM_001111020) Human Mass Spec Standard

CAT#: PH326321

SUPT5H MS Standard C13 and N15-labeled recombinant protein (NP_001104490)



  View other "SUPT5H" proteins (9)

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CNY 14,250.00


货期*
5周

规格
    • 10 ug

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Specifications

Product Data
Description SUPT5H MS Standard C13 and N15-labeled recombinant protein (NP_001104490)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC226321
Predicted MW 120.8 kDa
Protein Sequence
Tag C-Myc/DDK
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq NP_001104490
RefSeq ORF 3261
Synonyms SPT5; SPT5H; Tat-CT1
Locus ID 6829
Cytogenetics 19q13.2
Summary Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.[UniProtKB/Swiss-Prot Function]
Protein Families Transcription Factors
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