Renilla luciferase assay kit, 1000 assays

Specifications

Product Data
sds_url	/sds/pr300007.pdf
summary	This Renilla luciferase assay kit is designed to measure Renilla luciferase (RLuc) activity in transfected cell reporter gene assays. This kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.The substrate included in the kit is a water soluble substrate, aquaphile Coelenterazine, which can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine. The stated number of assays is based on 96-well assay format. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (Cat# PR300004) can be purchased separately.
note	This kit is a flash-type luminescence assay, which requires luminescence to be measured immediately after adding reagents to your sample.
_pt	2
components	• 5X Passive Lysis Buffer (30 mL) • Renilla Luciferase Assay Buffer 2.0 (100 mL) • Aquaphile Coelenterazine (1 X 4 mg)
_c	luciferase-assay-kit
sub_category	Luciferase Assay Kits
detection_method	Luminometer (single-tube or microplate reader with reagent injectors)
description	Renilla luciferase assay kit, 1000 assays
pdf_url	/datasheet/pr300007.pdf
storage	Store at -80°C.
product_name	Renilla luciferase assay kit, 1000 assays
product_manual_pdf	assets/documents/luciferase-assay-kit/renilla-luciferase-assay-kit.pdf
features	• Highly sensitive & linear flash-type RLuc assay • Easy-to-handle water-soluble substrates • Excellent performance & great value compared to other suppliers
assaytype	Luminescence (flash-type), Endpoint assay
background	Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
category	Others
stability	Renilla Luciferase Assay Buffer 2.0 is stable at -80°C for at least six months from date of receipt. Other kit components are stable at -20°C for at least six months from date of receipt. Kit components and stock solutions of Aquaphile coelenterazine in water are stable to at least 5 freeze/thaw cycles.
sizes_filter	1 kit

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.