Renilla luciferase assay kit, 150 assays
CNY 2,140.00
货期*
5周
规格
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Specifications
Product Data | |
sds_url | /sds/pr300002.pdf |
summary | This Renilla luciferase assay kit is designed to measure Renilla luciferase (RLuc) activity in transfected cell reporter gene assays. This kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.The substrate included in the kit is a water soluble substrate, aquaphile Coelenterazine, which can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine. The stated number of assays is based on 96-well assay format. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (Cat# PR300004) can be purchased separately. |
note | This kit is a flash-type luminescence assay, which requires luminescence to be measured immediately after adding reagents to your sample. |
_pt | 2 |
components | • 5X Passive Lysis Buffer (10 mL) • Renilla Luciferase Assay Buffer 2.0 (15 mL) • Aquaphile Coelenterazine (3 X 200ug) |
_c | luciferase-assay-kit |
sub_category | Luciferase Assay Kits |
detection_method | Luminometer (single-tube or microplate reader with reagent injectors) |
description | Renilla luciferase assay kit, 150 assays |
pdf_url | /datasheet/pr300002.pdf |
storage | Store at -80°C. |
product_name | Renilla luciferase assay kit, 150 assays |
product_manual_pdf | assets/documents/luciferase-assay-kit/renilla-luciferase-assay-kit.pdf |
features | • Highly sensitive & linear flash-type RLuc assay • Easy-to-handle water-soluble substrates • Excellent performance & great value compared to other suppliers |
assaytype | Luminescence (flash-type), Endpoint assay |
background | Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. |
category | Others |
stability | Renilla Luciferase Assay Buffer 2.0 is stable at -80°C for at least six months from date of receipt. Other kit components are stable at -20°C for at least six months from date of receipt. Kit components and stock solutions of Aquaphile coelenterazine in water are stable to at least 5 freeze/thaw cycles. |
sizes_filter | 1 kit |
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