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Pdcd1 Mouse Gene Knockout Kit (CRISPR)
CAT＃: KN312990RB
Pdcd1 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Pdcd1 |
| Locus ID | 18566 |
| Kit Components |
KN312990G1, Pdcd1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGCAGTTGAGCTGGCAATC KN312990G2, Pdcd1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGTACCCTGGTCATTCACT KN312990RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACGGATA GCTGGGCTCT TGCTCCTCAG TAGTAAAGGA CTAAGGCATA GCTCAGGGCA TTCAAGGCCA CGCATGGCAG ACAAGGTAGG GGAGGGTCCA GCTTGCCCTC GCTGCGGCCA TAGGTACCAA AGCCAGGCCT CGACACCCAC CCTCCAAAGG GACAAGAGTC TGGCCCTAGT TTCAGTCTCT CTCAGCCCTG GGAGCTAAGG CTCGATCGGG GTACCAGGAA TGGAAAAGAC CAAACCTACC CACAAGAGGG GCTAGAAATG GAGAGGACCC CATAGCAGGA CAAGAGGCAA GGACAGCTAG TCAGAGAGAA CCCCCCCTCT CTGCTCCCCA ATCTCTCACT AGTCCCTTAC CTGCTCCTCC CAGGCATCGT TCCCTCCCAC TCCCCTCCCC CTTCCATGCC CCTCCCCCAC CTCTAGTTGC CTGTTCTCCC ACCCTTGTGG AGGTGGAGGA AGAGGGGGCG GGAGCCAAGA ACAGGTCTCC TCCCTCCAAC ATGACCTGGG ACAGTTTCCT TTCCGCTACA GACAACTCTG CCTGAGCAGC GGGGAGGAGG AAGAGGAGAC TGCTACTGAA GGCGACACTG CCAGGGGCTC TGGGCCACCC TCGGTTCCTA TTACTGGAGG GGAGGAACAC CTGTGTCCCT GGTTACACGG ATTGGCTCCT GGTTACACAG ATTGGCTTTA GGTGGGTTTT ACAGGTGAAA TGCCCCCTGG GGCTCAAAAT CCACCCCCTC CTTGCTTTTA GACAGACAGA ACATTTATCT AACGTCAGTT GATCTTCTGT GTCCCTCTCT GTGAAGCGAT GGTAGGTCTG AACAGAGCTC TGACCTCAGC CACTGCTTCA GGATTCTGGC AGGCCCTGGT ATCCTTGGCC TTTCTCTATG TTTTTCTATG GTTGGGGACA TCCCTACATG GAGCCTGCCC TACACACTGT ATAGATGACT GAAGATCCAA GAGTATAATC AGCTGTCTAC CCTCATGGAC AAGCTTCCCG TGCTAGCATT TCTGGGGGAC TCCCGAGTTC ATATCCCTGG CCTCATAGCA TCCTGGGGGT GGGGGGTGTG TGAAACGGAG AGGTCCAGGA ATCCTGCTCA AATTCCTCAC AGTGAAAACA CAGCTCCCTG TACTCCTTAC TCCTCACTAC CCTGTACCCC TTGGCCCCTG CCCACCCTCT GGACTGAGCC AACCTTCACT GAGAATCACT CTCGCCGGTT GGACTTTAGA TCAGAAGGGA TCTTGCTGCC GCCCGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATCGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_008798 |
| Synonyms | Ly101; PD-1; Pdc1 |
| Summary | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11698646, PubMed:11209085, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:22641383, PubMed:18287011, PubMed:18641123). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).[UniProtKB/Swiss-Prot Function] |
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| 基因表达相关资源 |
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| KN312990 | Pdcd1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN312990BN | Pdcd1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN312990LP | Pdcd1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN512990 | Pdcd1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
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| GA203135 | Pdcd1 CRISPRa kit - CRISPR gene activation of mouse programmed cell death 1 |
CNY 12,255.00 |
