Gper1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN307164

Gper1 - mouse gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (2)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Gpr30 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 30 (Gpr30)
    • 10 ug

CNY 3,656.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol Gper1
Locus ID 76854
Kit Components

KN307164G1, Gper1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGATCTACCTAGGTCCCGTG

KN307164G2, Gper1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCCCCGGGGAACCTCACTG

KN307164-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TTGAGAAAGG CTGGGTTTTT CCCTTCCCAT GTCACGTCTC TTTCCAACAG CTGCTGAAGT TAATGAGACT TCCTGTCTCG ACAAACAGTA GTTTTCCTCA AATTCCCACA CCACACACTC ACGGTATTTC ACGGGCCAGT GATCTGACTG GTCCCACTAC ATCTCCCATG ACTCACAGAC TGTCACCAAC TGTGGGCGCT CTTAGGGAAC ATAAAGGAAC ACTCCACGTC TATCCACGTC TATCTCTTCT GCTCCAGCTC AGGGGGGTGG CGAAAGCCAG AGTCCAGGTG GACCCCGTTG TCACTCATGC ACCACACCTG GCTCCTTCAG GATGCTCAGG GTTCTAGATG CTAAGTCTTG CCCCGTCTGC TTCTACAGGT GCCCAGAAGG TGAGCAGGCA GCAGGTGTGC CTGCCCAGCA CCAGCCCAGA CATCAGACAC CCTGTCCACC CTTCTGGTTT TCTGAGACTA ACAGGCTCCC AGGACGATTC TTCCTGCCTC ACAAATGCCT GGTTTATCTT TCTTTGTGAA GATGGAGCTG TCACATAAAA CAGCTTTCTG TGACCCTTTC AGCAAATCCT GAAAACTGCC GAGGGAAGCC ATCAGCAGTA CGTGATTGCC CTCTTCCTCT CCTGCCTCTA CACCATCTTC CTCTTTCCTA TTGGCTTTGT GGGCAACATC CTCATCCTGG TGGTGAACAT CAGCTTCCGG GAGAAGATGA CCATCCCAGA CCTGTACTTC ATCAACCTGG CGGCGGCCGA CCTCATCCTG GTGGCTGACT CCCTGATTGA GGTGTTCAAC CTGGACGAGC AGTACTACGA CATCGCAGTG CTCTGCACCT TCATGTCCCT CTTCCTGCAG ATCAACATGT ACAGCAGCGT CTTCTTCCTC ACCTGGATGA GCTTCGACAG GTACCTAGCG CTGGCCAAGG CCATGCGCTG TGGCCTCTTC CGCACCAAGC ACCACGCACG GCTCAGCTGT GGCCTCATCT GGATGGCCTC AGTGTCCGCC ACGCTGGTGC CCTTCACAGC GGTGCACCTG CGGCACACGG AGGAGGCCTG CTTCTGCTTT GCTGATGTCA GGGAGGTGCA GTGGCTGGAG GTCACACTGG GCTTCATCAT GCCCTTCGCC ATCATTGGCC TCTGCTACTC CCTCATCGTG CGAGCCCTCA TCCGGGCCCA CAGGCACCGC GGCCTGCGCC

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_029771
Synonyms 6330420K13Rik; Ceprl; CMKRL2; FEG-1; GPCR-Br; Gper; Gpr30
Summary G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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