Ccdc50 Mouse Gene Knockout Kit (CRISPR)

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACCCCAC TAATCCCAGA TATAAGGATT TGTGGTGCCT GGCACTCAAT AAGCACTTTA CACAAGTCTT AAATCTCAGA GTGGAGCGCA GATAGGCTCT GTCCCGGCGG CGCCTAGCTG GCAGGTGCAG CCTTGGGGTC CCGGCCTTCA GGCCGCAGAG CTGTGGCCTG GGCTGGGCGC GGCAGGCACA TAGGCGAGGG GAGGCGGTCT GGCTCGCACG CCTCTGCGTG CGGTGCAGCC TTGCAGCCCC CGCCCCCCGC CGCCGCCGCC GCCGCCTTTC TGGCACCTCT CTCCCTCCGT ACTGGACTCC GGTGCATTTC CGGCCCGGGA GAGTCCGGCG TCCACCCGGG CCAGCCCGCG GGGTGGGCGT GCGCCGTGAT CTCCGCGCGC CCGAGGCCCA GCGGCCGCCC CTAGCATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA GAATTCCGAT CATATTCAAT AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG TTATTAGGTC TGAAGAGGAG TTTACGTCCA GCCAAGCTTA GGATCTCGAC CTCGAAATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT AGCAGCCCCG CTGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGC AGCTGGGAAG GGTGGGTCCG GGGGCGGGCT CAGGGGCGGG CTCAGGGGCG GGGCGGGCGC CCGAAGGTCC TCCGGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC TCGAGCAGCT GAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCACAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATG ATATCAGATC CCCGGGATGC AGAAATTGAT GATCTATTAA ACAATAAAGA TGTCCACTAA AATGGAAGTT TTTCCTGTCA TACTTTGTTA AGAAGGGTGA GAACAGAGTA CCTACATTTT GAATGGAAGG ATTGGAGCTA CGGGGGTGGG GGTGGGGTGG GATTAGATAA ATGCCTGCTC TTTACTGAAG GCTCTTTACT ATTGCTTTAT GATAATGTTT CATAGTTGGA TATCATAATT TAAACAAGCA AAACCAAATT AAGGGCCAGC TCATTCCTCC CACTCATGAT CTATAGATCT ATAGATCTCT CGTGGGATCA TTGTTTTTCT CTTGATTCCC ACTTTGTGGT TCTAAGTACT GTGGTTTCCA AATGTGTCAG TTTCATAGCC TGAAGAACGA GATCAGCAGC CTCTGTTCCA CATACACTTC ATTCTCAGTA TTGTTTTGCC AAGTTCTAAT TCCATCAGAA GCTGGTCGAG ATCCGGAACC CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCCCTC GAAGAGGTTC ACTAGGCGCG CCACTTCTTC TTCCTAGTCT AGTGTCTGGC TGGCTCTTAG CTTCAAAAAT GTTGAAAATC TTAGGGTTGG CATTGCTTTG AATGGTGGAC CTCAAGCCCC TATAAAGATG ATCTCCCCAC TTCCATTTTA ATAAAGACCA GGCTGGAGTG GGTGGGAGCT TGTGATCCTG TTACCCCCGT TGCCCCCCAT GGCTGCTATA TTGAAGTCCA GGAAAATAAG ATTGACTAGT ACTGGCCAGG CCTAGCTTAC TTCATCTCTC CTCCCCTCCC CGGGGCCTAG CATTTTTATT GTTTTGGTTA CTGGCTCATC TTCTTGTCAC TCTGTCTTTC CCTTCTTGGG AAGCACTGGA CTTCCATTAT GACAACACCT AGAACGTTCA AGAGTGCTCT CTCTTAAAGC CATAAGTTCT GAGGGGGGTG GGGGGGGGGT CTTTGTTGTG GATCTCTTTT GTTTCACCAG AGATCTGTGG CCAACACGGT CTGGGACTGG GATGTGTGTG TTGTGTAAGT GAAGGAGTTT TTCCTTTCTT GAGACTTTAG GCTTCATTGC CAATTTCAAG TGACTCAGAT GGTCTCGCTC TGGCCAATTT GGAAATCCCA AGTCACTCTC GCCGGTTGGA CTTTAGATCA GAAGGGATCT TGCTGCCGCC CGAAAGAGGA AGGGCTGGAA GAGGAAGGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATC