Ankrd1 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Ankrd1
Locus ID	107765
Kit Components	KN301262G1, Ankrd1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTAAGAGAAGGTGGTTGAG KN301262G2, Ankrd1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTTTCTCAGGAATAATGTG KN301262-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAGACATCAC TCCAGAGAAT TCCAAACAGA TAGAGACAAG TGGCACCCAG ACCCATCTCC TTCCCCTCGG GCTGATTATC CCCAGAAATA GGATGTCCCA AAGCAACACT TCCCAGCCAA CTGGAGTGCT GATAAGTCCA GTTATCAGAA AGATATGGCT GTAAGTGTGA TGCACAGTGC TTGCATTTTC TTGATACGTT AGTCATATGA GAGCTGACAA AGAAGGAAAA AGAGCAGCGA TGTGGTGCAA TATTAACAGG CAGCTGTCCC CTGGCTTCCC GATACGTGGG ATGACTCGCA TTGCTGAGCG GTGTGGTCAC TGCCAAAGGA ATGACCCTCT CACATTTCTT CCTGATTCGC ATACGCCGCG GCCAGCTTGT CATCTCCCTC TTGGGCTTCC CAGACACTAA GTCTGGAATG AAAATTCACC TGCCTCTGAA TTGGCCACTG GTGGGGGCAG GGGTGTGACT TGGCTTCCCA GGCTGGAAGA TTATCTCACC CAGCCCTAGC TATATAACGG GCTGGTGTGG AGGGGCTCCA CAGGGCCAGT TCCAGGGGTT CATCCACAAG AGAGAAAAAC ATAGACTCAC GGCTGCCAAC TGATGCTCAT CTTAATTGTG AGAGGAAGGC AGGCACCTGG GATACCTGGA GCTATGCTCC AGACAGTGCA CCGCCTGAAG GTGGCTGTCG CTGGATGGAG AGGAAGGACC TCCTGTGGCA TTGTTTGTGG ACAGTTACCC CAAGATGGGT AGCTTTGCTT CTGACTTCAA CATCTTCTTC ACATACAGGT AACAGGCAAA AAGAACAGCA ATGGGGCCGC AGGGGAATTC CTTCCTGGGG AGTTCAGAAA TGGAGAATAT GAAGCTGCTG TTGCTTTGGA GAAGCAAGAG GACCTGAAGA CACTTCCGGC CAACAGCGTG AAGCAGGGGG AGGAACAACG GAAAAGCGAG AAACTGCGAG AGGCAGAGGT GAGGACCTCC CACAAAATCC TAGTGGGAGT GGGGTACGGA GGTCCCTGAG GAAGAGGGGA AGAGAGGAGG AAAACAAATA AAAACTGCTC TAAAGATGTC TTTTCACCAG ATATGAAGCG CATGATGCAA AGAAGCAGTG CACATGGAAA TGACTGGGGG AATTACTTTT GGATTCCTCC TGTGTGGAGG GTTGAATCAA TGATTGAATG TGCTATGACC ACGTTTTGAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_013468
Synonyms	Alrp; CARP; Crap; MARP1
Summary	May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.