CHD1L Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CHD1L
Locus ID	9557
Kit Components	KN223499G1, CHD1L gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCATACTGAGGGCCGAGCCG KN223499G2, CHD1L gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGCGCGGGCGCTACTAGCCG KN223499-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CCTCAATCTG CTCCCCATAA TGCACTTGAG ATACCCACTG CCACAATGAC GTCTTTGGTG ATTTTAATTT ACTACTATTC AGAGGTTCCC AATTTTGGCC TACAGGTCAC AGGCAGTAAG AAAGGGATTT CAGGGACTAA ATCAATTTAT CACTAGGTAA ACCGAACAAG AACAGTTTGA TCTTATCGTG AATCACTGGA GGGCACGGAA CAAGTCTTAC TTCTAGAACC CCCAGGGCCC AGCGGAGTGT CTGACACATG ATAGTCGATG TTGATTAAAT AATAAATGAA TGATGAACAC ATGCCACCTC CTTGACGGGC AGTTCAGGCA ATCAGACGTC CTGTGCGTGA TCAGACGCCT AGCGCTTCAG AAGCACGACT GGTGAGAACT GGGCAGCCCT TCCACGCTCC GCACTGCAAG AACTGCCTGT CCGGAAGGGT GACTGCACCA GCTCCGCTCC GGATATCTTG GCAGGACGCG CCCTCTGGCG GCGCCTCGCT CGTAGGTGGG CCCCAGCGCG CAGTCGCGCG CCCCCGCGCG TTGGGAAGTT GGGAGGGAGG TGCGCGCTTG GCCGCGCGGG GCGGGGCCTC TACCGGCCCG GGCGGCGCGG GTGCAGGAGC AGGACTTACG GCAGTGGGGG CTGACAGGTG AGCGGGCTCC GGGCGGACTT CGGCCAGGCG GGCCAACCTA TTGGGACTGC CTCTTGCGGG CCGGGGGCGC AGCGGGTGGG CCGCCCGGTG GGCAGTTTAC CCGCTCGCGC CAGGGCCTTC CTTCGCTCCT GCCGTGGGCG GAGAGAAACT GCGCCCGGGC TTGGTGGCCA CGGCCCCCGC CTGCGCGGCG CGCGGGGCCG GCGGACGCCA GATGAATGAG GTCGCGGGCC CGGTAGCCAG GCCTGGCCCC TGGCCTGTGG AGGGGAAACG TGGTGAGATC AGCGCCACCT GCAGGCCTCC GCTGAAGAGT TGGACGGCCT GGTGCACCAA GGATGTAGGT TTCCAGTTGC TGTTCCGGTG GGTTTGGGTC CCGGCCTGGG GAGTTTATTG GTTGCTTCTG TAAAACGATC AACTCATCTC CCAGCTTCAG CTTCTCCGTT TACTGTGCGC GCGCAGTTAT TGGAGAATTC GCCACGGATT AATTCTCCGG ATCGAGTTAC GTGCGGTAGT GAGCGGACAA ACTCAAGCAA AGCACTGTTT ATGAAGCTAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001256336, NM_001256337, NM_001256338, NM_004284, NM_024568, NR_046070, NM_001348451, NM_001348452, NM_001348453, NM_001348454, NM_001348455, NM_001348456, NM_001348457, NM_001348458, NM_001348459, NM_001348460, NM_001348461, NM_001348462, NM_001348463, NM_001348464, NM_001348465, NM_001348466, NR_145681, NR_145682, NR_145683, NR_145684, NR_145685, NR_145686, NR_145687, NR_145688, NR_145689, NR_145690, NR_145691, NR_145692, NR_145693, NR_145694, NR_145695
Synonyms	ALC1; CHDL
Summary	This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Overexpression of this gene has been linked to several types of cancers. [provided by RefSeq, Feb 2017]

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