CHD7 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CHD7
Locus ID	55636
Kit Components	KN221060G1, CHD7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGAGTCTTTTTGGCGAGGAT KN221060G2, CHD7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTCGGAGAATGTGGTTACC KN221060-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGGTTAAGG GCGGGCTGTT GGTAACCAGA GCATTTCCAG GGCTTTAGTT CAGCAGATGA GCTGCTTGGC TATATTCATA CATCTGCTTG CCTGCTGTGG CAGCTGCTGC TGCTTCACGT TGTGTTCTTT TGTTCTGTTT CATCCAAGAA TGGAACGCTA GGAAGTGTAG TGAATGGATT AATTTAAGGT TAAGGTTAGT GTTTTGCTTG CTTTCTAAGT AACCGATCAT AATGATGCTT CTGGTAGAAG CTAATGAGTT TCTCTTAAAG TGAACTAAAT TTATAAAATA CAGCTCAGCG AGGGAGCTAG ATTCGTATTG TTGATGGGGA CCAAATTCAG ATGTACAAAA TACTGGCAGT TTTTATCATT GGATCCTAAT TCTTTGTTTT CTTCCTTCTT CTCCCCCACC CCAAACTCCC TTCCAGGACC TATATCCAGA CTTTGCCTGA CACTGCAGGG TCCAAGAGAA TTAAAGAAAT ATGGAATGAC ATGAAGAAGA TTAGTTAAGG ATTATAGGCT TTGAGGGCAA ACACCTCAGT GAAGTGAAGC ACAGGCAAGC TCCTGAGCTG TGGTTTGGAG GAGCCGTGTG TTGGAAGAAG GAAAATCCAG TAAATCCTAT GGGTCAGCAA ATGCCAATAG ACCAAGGCTT TGCCTCTTTA CAGCCATCCC TTCATCATCC TTCAACTAAT CAAAATCAAA CAAAGCTGAC ACATTTTGAT CACTATAATC AGTATGAACA ACAAAAGATG CATCTGATGG ATCAGCCGAA CAGAATGATG AGCAACACCC CTGGGAACGG ACTCGCGTCT CCGCACTCGC AGTATCACAC CCCTCCCGTT CCTCAGGTGC CCCATGGTGG CAGTGGTGGC GGTCAGATGG GTGTCTACCC TGGCATGCAG AATGAGAGGC ATGGGCAATC CTTTGTGGAC AGCAGCTCCA TGTGGGGCCC CAGGGCTGTT CAGGTACCAG ACCAGATACG AGCCCCCTAC CAGCAGCAGC AGCCACAGCC GCAGCCACCG CAGCCGGCTC CGTCGGGGCC CCCTGCACAG GGCCACCCTC AGCACATGCA GCAGATGGGC AGCTATATGG CACGTGGGGA TTTTTCCATG CAGCAGCATG GTCAGCCACA GCAGAGGATG AGCCAGTTTT CCCAAGGCCA AGAGGGCCTC AATCAGGGAA ATCCTTTTAT TGCCACCTCA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001316690, NM_017780, NM_017783
Synonyms	CRG; HH5; IS3; KAL5
Summary	This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

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