BORIS (CTCFL) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	BORIS
Locus ID	140690
Kit Components	KN216042G1, BORIS gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTCGAGTTGATGCCGGAAAA KN216042G2, BORIS gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTTGGTGAATTGCTCAGAA KN216042-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTGGCCAAGT CTGGGTGGGC TCGAGGCCAC TAGGCCCAAA GCCTGCCTGG CTCTGAGGGT GCTAGGTCTA GAACCGTGCA CGAGGGGAAT GCCTGCTCGG GCCCGAACCT CGCTGGGCGC CGGGTGTGCA CTGGCCCGGG GCCTGCTTGG ACCTGAAACT TGCTAGGCCC AGGATATGCA CTGGCCGAGA GCCTGCTGGG CCCAAACCTT ACTAGGCCCA GGATGTTCAC TGACTGAACC GGCTCAGGCC TAACCTTGCT AGGCCCAGGA TATGCACTGG GCCAGAGTGT GCTCAGGCGG AACCTTGCCA GGCGCAGGAT GTGTGCTGGC CCTAAGCCTG CTGAGGCCCA AACCTGTTCG TTCTAGGGTT TTGTACAAAA TCCTGCTTTA GCCTAAATCC TGCTTAGCCT TGACCCCCTC CTAGACCCAA GCCAGATCAG CATTGTTCTG ACCCTACTAA GTCCAAAACC TTTTGAGGCC AGACCTTGTT TCAACTCCAA AGCCTGCTAG GTTCCAGCAC CCCCCGCATC CCTCCTCATA CCACCCCCTT CTCCCCCCTA TGGAAACCGC TTGCTTATTT TTCAAACAGG CCAAGTCATT AAAGACCATC GGAGCCCTAG TGAGTTGGAG GCCGAGCGTA CCTCTGGGGC CTTCCAGGAC AGCGTCCTGG AGGAAGAAGT GGAGCTGGTG CTGGCCCCCT CGGAGGAGAG CGAGAAGTAC ATCCTGACCC TGCAGACGGT GCACTTCACT TCTGAAGCTG TGGAGTTGCA GGATATGAGC TTGCTGAGCA TACAGCAGCA AGAAGGGGTG CAGGTGGTGG TGCAACAGCC TGGCCCTGGG TTGCTGTGGC TTGAGGAAGG GCCCCGGCAG AGCCTGCAGC AGTGTGTGGC CATTAGTATC CAGCAAGAGC TGTACTCCCC GCAAGAGATG GAGGTGTTGC AGTTCCACGC TCTAGAGGAG AATGTGATGG TGGCCAGTGA AGACAGTAAG TTAGCGGTGA GCCTGGCTGA AACTACTGGA CTGATCAAGG TACAGCTGGT TTTAATAAAC AACCCCTTAC ATGAAAAAGA AAAAATTCAA GTCGGCTTTT ATGCAATATT ATGTCTGGAC AAGGTATTTT GCAGTTGCAA ATTAAAGACA GTACAAATAC TTCCCAGGAA AATCTTGAGA GTTCTTAGGT TCTTTTTTAA AAAAGAGTTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001269040, NM_001269041, NM_001269042, NM_001269043, NM_001269044, NM_001269045, NM_001269046, NM_001269047, NM_001269048, NM_001269049, NM_001269050, NM_001269051, NM_001269052, NM_001269054, NM_001269055, NM_080618, NR_072975
Synonyms	BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
Summary	CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

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