Neurexin 1 (NRXN1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Neurexin 1
Locus ID	9378
Kit Components	KN215381G1, Neurexin 1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGGCGCTGCTCCAGCGCG KN215381G2, Neurexin 1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGCCTCTCGCTGCTGCTCC KN215381-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GCCCTAAAGA GGCAAGCATC CCTTTCTCCG TTCTGCTGTA AGACACCACA TCTGAAGGAG ACAGACTTGA GGCATTCACA AGCATCCAAG GATACTGGCT CACTGCTGGC TTCCTGTACT TTCTTGAGAA AAGTGTTTTC TTTTTACTGA CTCTCACTGC TTCCTTTTGG CCTGGTACCA CTGGTGGAAC CTGGCAGATA ATGGGATTTG AGGAGTTAAG TATCTGTTGA CACTGCAGAT ACTCTTAAAT TAGTTAATCA GACTCAAATG GGTCAGTGCT TTCCCTTGAT CTTTTGTTGA AGCCTTTTCC AGAACGTTGT CTCCAAAGTG CATCCTCCCT TTGGTCTGGA AGTTCTGTGG CTACCCTGTA CCACTGGGGT CAACGTTTTC CAGTTGGAAG AGGACTACAG GGAGAGAGGG CGGGGTTATT GGAAGAAGAA GAAGAAAAAA AGAACTAGAT AAAGGAGGGC ACATCCCTCT CACTCCCGCC TTTCCCCTTA CCTTTCTGTC TCTCGGGACC CTTATTTCTT CGTCACGGTG TCCAGGACCA TTTTGACCCT GTCGGCCCCG GCACCCCCCC GCCGCACCCC AGCCCCGAGC GGCAGCGGGC TGGAGTTTCC GGGCGCCGAG GGCCAATGGA CGCGCTTCCC CAAGTGGAAC GCCTGCTGCG AGAGCGAGAT GAGCTTCCAG CTCAAGACTC GCAGCGCCCG CGGCCTCGTG CTCTACTTCG ACGACGAGGG CTTCTGCGAC TTCCTGGAGC TGATTCTGAC GCGCGGCGGC CGCCTGCAGC TCAGCTTCTC CATCTTCTGC GCTGAGCCTG CGACGCTCCT GGCCGACACG CCGGTTAACG ACGGCGCCTG GCACAGCGTG CGCATCCGCC GCCAGTTCCG CAACACCACG CTCTTCATCG ACCAGGTGGA GGCCAAGTGG GTGGAGGTCA AGTCCAAGCG CAGGGACATG ACGGTGTTCA GCGGCCTTTT CGTCGGGGGG CTGCCCCCGG AACTGCGCGC CGCGGCGCTC AAGCTCACCC TGGCCTCGGT GAGGGAGCGG GAGCCCTTCA AGGGGTGGAT TCGTGACGTG AGGGTCAACT CCTCGCAGGT CCTGCCCGTG GACAGCGGCG AGGTGAAGCT GGACGATGAG CCGCCCAACA GCGGCGGGGG AAGCCCGTGC GAGGCGGGCG AGGAGGGCGA GGGCGGGGTG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001135659, NM_001320156, NM_001320157, NM_001330077, NM_001330078, NM_001330079, NM_001330081, NM_001330082, NM_001330083, NM_001330084, NM_001330085, NM_001330086, NM_001330087, NM_001330088, NM_001330089, NM_001330090, NM_001330091, NM_001330092, NM_001330093, NM_001330094, NM_001330095, NM_001330096, NM_001330097, NM_004801, NM_138735
Synonyms	Hs.22998; PTHSL2; SCZD17
Summary	This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia. [provided by RefSeq, Aug 2016]

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