GRK5 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	GRK5
Locus ID	2869
Kit Components	KN209137G1, GRK5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAAAACATCGTGGCCAACA KN209137G2, GRK5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTCTTGCTGAAAGCCAGGGA KN209137RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet GCCGCCTTTG AGTCGCCTGT CGGCCGGGCG GTGCCCGCCT TCGCTTTCGG TGCCGTCCCG GGGCGGGTCC TCGCTCCGAC AGCCGCGCGC CAGCCCAGCT CAGCCCCGGC GCCGCGCGGC CGGGAGGACT CCAGCTCCCA GCAGGCCCCG CGCCGCGAGC ACCGAGGCAG GAGGCGCGCC TGGGCCGGCG CTTGCGCAGT GCGGCCGCCC GAGCCTGCTA GTACCACACC CCCGGGAGGG ACTGAGGGGA GGCAGAAGCA TCCGAGGCAT TAAAGCATCC GAGGGAGCCG GAGGGGAGGA GAATGGAGTG ACAGAGACAC GCGGAGGGTG GGGGGTGGGG GGGAGCGTGT TGAGGGAGGG GGGAGGGGGG ACACAGAGGG AGGAAGAAGC GGCGGCGGCG GCGGCGGCGG CGGCGGCTCC TCTTTGCAGA GGGGGAAACT CTTGGGCTGA GAGCAGGAAT AATGCGGTAG GCAAGGCGGG CTGCTGGCTC CCCCGGCTCC GGCAGCAGCG GCGGCAGCCC GAGCAGCGGC AGCAGCAGCG GCAGCACCCC AGGCGCTGAC AGCCCCGCCG GCCGGCTCCG TTGCTGACCG CCGACTGTCA CCGGTACGTG CCCGGCGCGT CCGCCGCGGG CCAGGGTGCG GGTGTCGGGT GGCGTGCGGG CTGGGGCTGC GCCCGTGCAG GATGCCCGCT GCCGGCGAGT GGGTCGCGAG CAGGACACTT CGGAGAGCGG CTCTGCAGAC CCTTCAGGGT TCCTGCCTTG GGGCTCAGAG AATCTGGACT TGGGCTGAGA AGGTTGCAGG GATTTGGATG CTAACATATC TCCTAGTTTC CAGCGCTCGC CCTGCAGAGC GTGCCATCTC GGCAGCTGTT GAAAGGTCAG TTGGAAAAGC ATTCATTCGG GGAGCGACGA GCAAACATGC ACATAAAATA GCGTTTCAAG TCGGCCCGGG ATGGTTCTGT GTGAACTGGT GTGTGTGTTC GAGGGGCGGG GGTGGAATGA GCAGAAGCCA AGTCTTTGCC TGCGCAGGCT GAGTTGATAT AGACTATTGC TGGAAACCAT CCGCTGGGAT GTTTCTTAGG GAAATCCCGT CGTGTGGAAG GAAGCCTTTT AAAGGCAAAG TCAGATGTTG AGGAATTAGC CTTTTCATAG AATTATCAAA GGATGGCTGT ACAGACCACC TAGAAAATAA TTCAGTATTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_005308
Synonyms	GPRK5
Summary	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

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