CD146 (MCAM) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CD146
Locus ID	4162
Kit Components	KN208937G1, CD146 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGGTGAGTTCGCTCGCAGGG KN208937G2, CD146 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCACCGCAGACCCCTAGCCG KN208937-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCTGTGGGAC TGGCGCAGGG TTCTTCTCCC AAAGCTGCCA GCACTGAGGC TGAGGCAGTG TCAGGCCGGC GGCAGCGGCA GTGGTGCAAT CGTTCTGGGA AGCGATAGAG GCCGGCCTGA ATTCTCTGTG GCAAGGGAGG GGAGCCCAAG TGGGAGGCCC CTTGGGGACA CCGAGGACCA GGTCCGCTAC TGCTCCTCCC CCAGGAGGTC CCCTAGGGGC TACATTGGCT GGCAGGGGCT GAGCAGCGGT GAGCCTGGCT GGCTTCGACC CGGGGCGACT CCGGGCATCC GGGACAGCTT CTCCTCGCTG CCACCTCGGC CAGTCAGACC CCGAGACACC TGTCACTACC CCCTCAGCCT TCCCAAGCCA GGAGCCTGGG AGTCCGGCTC TGGCCTACCT CCGGCAGCGC TCCTAGGCGC AGCTCCCGGG CTGGCGGCGC CGGGGCCCGC CCCCTAGGGC TGCGGCGCGC GGGGCGGGGG CTGGGGGCTG CGCGGGGCGG GGCGGGCCCG GGCGCTCCGG GCCCCCTCCC CCGCCCCCCT GACGTCAGCC CCCGGCAGCC TCGAGCTGCT CACTTGGCTC TCGCCCTCCG GCCGGGAAGC GCGCGGCGGG GTCGGGGCGT TGCGTCTGGG AGGACGAGCC TCCTCCCTGG GTCCCCGATC CCCGGGCGCC TTGCGCGCGA GCAACTCTTC TTTGCAGCCA GTTTGCAGCC GGGATTCTAG AGTATCCCGG GAGCAGCACT CGGAAGGCGG GGAGGAGGCT GCTTCTGGGA ACGAGAAGGG GTGGAGCTCA GCCTTTCGGG GTGCTGGGGG GTGGGTGGTC CCTGAGGTGC TCACTCTGGG GGCCCGCAAT TGAAGCCGGG CAGGAGGCGC AGCTGGGGCG CATCCTCAAA GCCTGAATTC CGCGCCCGCC TGTTGCTGGA AAAGGCAGCT TCCTTCGCTG GAGGGGGTGC GCCGACCCAC CCCTTCCCCC TTCTGCCTGG GCATCACGCC AGGCTGGAGG TGAGCGAGAG CGGGAGGTTC GGCGGCTCCC GCCCGAGCTG GGCGTTGGCA GGGGTTGCGG GGCGGTGTGG GTCGCCTCGC GCCTCCCCGA GTGATGGGAT CATAGGGGAC AGAGATGAGG GATGGAGGAT TCCCATACTG GAAGCGCCCG CTGGCTTATT TTGGGGACCA CATTCAGGTG GGAAGTGCGC CCGGGCACCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_006500
Synonyms	CD146; MUC18
Summary	Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.[UniProtKB/Swiss-Prot Function]

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