YY1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	YY1
Locus ID	7528
Kit Components	KN207198G1, YY1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAGACCATCGAGACCACAG KN207198G2, YY1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGAGGTGGAGACCATCCCGG KN207198-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAGGGCGCGT GCGAGCCGCC CCGCCCCCGG TCCCATCGGC CCCAATCCGG GAGGAGCCCG GCGAGTGGGC GGGGCCGCGG AGGCCAGCGG ACAGATCGAT TGGCCGAGAG GAGAATCGAG AGGGCGAACG GGCGAGTGGC AGCGAGGCGG GGCGGGCTGA GGCCAGCGCG GAAGTCTCGC GAGGCCGGGC CCGAGCAGAG TGTGGCGGCG GCGGCGAGAT CTGGGCTCGG GTTGAGGAGT TGGTATTTGT GTGGAAGGAG GCGGAGGCGC AGGAGGAAGG GGGAAGCGGA GCGCCGGCCC GGAGGGCGGG AGGAGGCGCG GCCAGGGCGG GCGGTTGCGG CGAGGCGAGG CGAGGCGGGG AGCCGAGACG AGCAGCGGCC GAGCGAGCGC GGGCGCGGGC GCACCGAGGC GAGGGAGGCG GGGAAGCCCC GCCGCCGCCG CGGCGCCCGC CCCTTCCCCC GCCGCCCGCC CCCTCTCCCC CCGCCCGCTC GCCGCCTTCC TCCCTCTGCC TTCCTTCCCC ACGGCCGGCC GCCTCCTCGC CCGCCCGCCC GCAGCCGAGG AGCCGAGGCC GCCGCGGCCG TGGCGGCGGA GCCCTCAGCC AGGAGGAGGA CGACGACGAC GAGGACGGCG GCGGTGGCGA CCACGGCGGC GGGGGCGGCC ACGGGCACGC CGGCCACCAC CACCACCACC ATCACCACCA CCACCACCCG CCCATGATCG CTCTGCAGCC GCTGGTCACC GACGACCCGA CCCAGGTGCA CCACCACCAG GAGGTGATCC TGGTGCAGAC GCGCGAGGAG GTGGTGGGCG GCGACGACTC GGACGGGCTG CGCGCCGAGG ACGGCTTCGA GGATCAGATT CTCATCCCGG TGCCCGCGCC GGCCGGCGGC GACGACGACT ACATTGAACA AACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGCGGC GGCGGCTCGT CGTCGTCGGG AGGCGGCCGC GTCAAGAAGG GCGGCGGCAA GAAGAGCGGC AAGAAGAGTT ACCTCAGCGG CGGGGCCGGC GCGGCGGGCG GCGGCGGCGC CGACCCGGGC AACAAGAAGT GGGAGCAGAA GCAGGTGCAG ATCAAGACCC TGGAGGGCGA GTTCTCGGTC ACCATGTGGT CCTCAGGTGA GCGCCGGCCG CGCGCCCCGG CCCCGGGATG TTTTTGTTTT GAAGGGAAGC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_003403
Synonyms	DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1
Summary	YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]

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