Rb (RB1) Human Gene Knockout Kit (CRISPR)

CAT#: KN206933RB

RB1 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


RB1 mouse monoclonal antibody,clone OTI13A6
    • 100 ul

CNY 1,999.00
CNY 2,700.00


RB1 (Myc-DDK-tagged)-Human retinoblastoma 1 (RB1)
    • 10 ug

CNY 6,800.00

Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol Rb
Locus ID 5925
Kit Components

KN206933G1, Rb gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAGGACCCAGAGCAGGACAG

KN206933G2, Rb gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCGCTCACCTGACGAGAGGC

KN206933RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC CTTCCTCTTC CAGCCCTTCC TCTTTCACTG ACTGACTGAC TGCGTCTCAA CCTTTTGTTT TGGCCGACTT TGCAAAACGG ATTGGGCGGG ATGAGAGGTG GGGGGCGCCG CCCAAGGAGG GAGAGTGGCG CTCCCGCCGA GGGTGCACTA GCCAGATATT CCCTGCGGGG CCCGAGAGTC TTCCCTATCA GACCCCGGGA TAGGGATGAG GCCCACAGTC ACCCACCAGA CTCTTTGTAT AGCCCCGTTA AGTGCACCCC GGCCTGGAGG GGGTGGTTCT GGGTAGAAGC ACGTCCGGGC CGCGCCGGAT GCCTCCTGGA AGGCGCCTGG ACCCACGCCA GGTTTCCCAG TTTAATTCCT CATGACTTAG CGTCCCAGCC CGCGCACCGA CCAGCGCCCC AGTTCCCCAC AGACGCCGGC GGGCCCGGGA GCCTCGCGGA CGTGACGCCG CGGGCGGAAG TGACGTTTTC CCGCGGTTGG ACGCGGCGCT CAGTTGCCGG GCGGGGGAGG GCGCGTCCGG TTTTTCTCAG GGGACGTTGA AATTATTTTT GTAACGGGAG TCGGGAGAGG ACGGGGCGTG CCCCGACGTG CGCGCGCGTC GTCCTCCCCG GCGCTCCTCC ACAGCTCGCT GGCTCCCGCC GCGGAAAGGC GTCTCGCCTC ACGCGGGAAG GGCGCCCCGG GTGTGCGTAG GGCGGGCGCC AAGGCGGCTC GGCGGGGATC CGTCCTCGCC AGGGGCCGGG TCCCGGCGGG AGGAGGCGCC CTCCCTGCCC CCCGCCACGG CGGAGCGTCT GCAGAATGGT GACAGGATTC TGGGTTCTTG GGCGAGGGGT CTCGGCTTCA ACTTGACAGG TGTCGGGCGG GTGGGGCTAG GGTCCTGAGC GAAGTGACAG GTGCAGTTCC CTCTTGTGAG GCTCGGAGGC AGAGGGTCGT TGCGAGCGTC CATCAGACGC AAAAAATGAA AAATAAAAAT ACAAAAATGG TGTCTGTGGG AGAGTTTTTC ACCGGAGAAT TGGAGTACTC CGGTGGTCGT CTGACTTTCT GTTTTGGTTC ACGCGATGCA ACAGTTGGGA AGTATTTTCT TCCGGGCGTG CACTGCATCT GAAGTCCATT TGTGGGAGAG GCCGACCAGA AAGCCTTGGA CAAGAAGCGC AGGGTCCTGA GTGTCCATTG CCCACAGGAT ACTCGGCTCA GGAGCTTTGC GGCGTTTCCT TAGAACAATA ATGCATCGAG GCCTTGGGGA CTCAAAGCCA TCTACGAAGA GACGACTGAC TGACTGACTG GAAAGTCCTC TCCACTGACT GTAGCCTCCA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCAACGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGACCCGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTT

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000321
Synonyms OSRC; p105-Rb; pp110; PPP1R130; pRb; RB
Summary The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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