Angiopoietin like 4 (ANGPTL4) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	Angiopoietin like 4
Locus ID	51129
Kit Components	KN205651G1, Angiopoietin like 4 gRNA vector 1 in pCas-Guide CRISPR vector KN205651G2, Angiopoietin like 4 gRNA vector 2 in pCas-Guide CRISPR vector KN205651LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet GGACTACAGG CGCGCACTAC CAAGCCCGGC TAATTTTTTT TTGTATTTTT AGTAGAGACT GGGTTTCACG ATGTTGGCCG GGCTGGTCTG GAAGTCTTGA CCTCAAGCGT GCGCCCTCTC CGCCACTGGG TAAGGCGGGG GCGGAATAGG GGGCTTGCAA TTTCACACTA GAGGCGGGCA CCGTGGGGGA AAGAAGAGTC ACGTCTCCCA CGGTTCGTAG AGGAAGGCCT GCCTGAGCCT GGAGCGGGGG CGGGAGAGCC ACAGTTTGGC ATCCCCAGGG CATCCCCCAG CCCGCAGACT ACCAGGCCTC CAGAGGACAG GACCCCACCC CCGGCCACAG GCCCTGCCCC CAGCACTCCC CGCACCCCGC CTCCAAGACT CCTCCGCCCA CTCCGCACCC AACTTATAAA AACCGTCCTC GGGCGCGGCG GGGAGAAGCC GAGCTGAGCG GATCCTCACA CGACTGTGAT CCGATTCTTT CCAGCGGCTT CTGCAACCAA GCGGGTCTTA CCCCCGGTCC TCCGCGTCTC CAGTCCTCGC ACCTGGAACC CCAACGTCCC CGAGAGTCCC CGAATCCCCG CTCCCAGGCT ACCTAAGAGG CCTGCAGCTC GGCCAGGGGC TGCGCGAACA CGCGGAGCGC ACCCGCAGTC AGCTGAGCGC GCTGGAGCGG CGCCTGAGCG CGTGCGGGTC CGCCTGTCAG GGAACCGAGG GGTCCACCGA CCTCCCGTTA GCCCCTGAGA GCCGGGTGGA CCCTGAGGTC CTTCACAGCC TGCAGGTACG TGTCCCCAGG GCTGGTTCTC CGCGCCCCTA GTGGCTCTCC TGGCTTGGAA GGGTATGGAC AGGAGTGGGG CGTGGGGGCG GGGTGCGCAA CTGTGGCTCC CTGGGCTTCC CTGCGTCAAG GGATGGGCTC CCCCCTTAGG AAGCCGAGGA GGGAGGTTCG CTCAAGGCCA GGAATTCAAG ACCACCTAAA GCAAAATAGC GAGACCCCCG TCTCTTCTAC ACACACACAC ACACACACAC AAAATAAATA AAATAAAATA AAATAAATAT AAAATTAAAA AACGACCGGG CGCAGTGGCT CACGGAGCCT CCACCTGCTG TCGAGACTAG CCTGGCCAAC ATGGAGAAAC CTCGTCTCTA CTAAAAATAC AAAAAATTAG CCTGGCATGG TGTGCATGCC TGTAATCCCA GCTACTTGGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001039667, NM_016109, NM_139314, NR_104213
Synonyms	ANGPTL2; ARP4; FIAF; HARP; HFARP; NL2; PGAR; pp1158; TGQTL; UNQ171
Summary	This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]

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