Nrf2 (NFE2L2) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Nrf2
Locus ID	4780
Kit Components	KN204140G1, Nrf2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCGTCCCGGCACCACCGCA KN204140G2, Nrf2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGGGACGGGAGTCCCGGCGG KN204140-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CGGGCGGTAA AGTGAGATAA AAGCAGGGCA AGGTTCTGCA ACTCCAAATC AGGGAGGCGC AGCTCCTACA CCAACGCCTT TCCGGGGCTC CGGGTGTGTT TGTTCCAACT GTTTAAACTG TTTCAAAGCG TCCGAACTCC AGCGACCTTC GCAAACAACT CTTTATCTCG CGGGCGAGAG CGCTGCCCTT ATTTGCGGGG GAGGGCAAAC TGAACGCCGG CACCGGGGAG CTAACGGAGA CCTCCTCTAG GTCCCCCGCC TGCTGGGACC CCAGCTGGCA GTCCCTTCCC GCCCCCGGAC CGCGAGCTTC TTGCGTCAGC CCCGGCGCGG GTGGGGGATT TTCGGAAGCT CAGCCCGCGC GGCCGGCGGG GGAAGGAAGG GCCCGGACTC TTGCCCCGCC CTTGTGGGGC GGGAGGCGGA GCGGGGCAGG GGCCCGCCGG CGTGTAGCCG ATTACCGAGT GCCGGGGAGC CCGGAGGAGC CGCCGACGCA GCCGCCACCG CCGCCGCCGC CGCCACCAGA GCCGCCCTGT CCGCGCCGCG CCTCGGCAGC CGGAACAGGG CCGCCGTCGG GGAGCCCCAA CACACGGTCC ACAGCTCATC ACTAGCATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AGAATTCCGA TCATATTCAA TAACCCTTAA TATAACTTCG TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTT AGGATCTCGA CCTCGAAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGACTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CATCCATCTA GATCTCGAGC AGCTGAAGCT TACCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC ACAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATATCA GATCCCCGGG ATGCAGAAAT TGATGATCTA TTAAACAATA AAGATGTCCA CTAAAATGGA AGTTTTTCCT GTCATACTTT GTTAAGAAGG GTGAGAACAG AGTACCTACA TTTTGAATGG AAGGATTGGA GCTACGGGGG TGGGGGTGGG GTGGGATTAG ATAAATGCCT GCTCTTTACT GAAGGCTCTT TACTATTGCT TTATGATAAT GTTTCATAGT TGGATATCAT AATTTAAACA AGCAAAACCA AATTAAGGGC CAGCTCATTC CTCCCACTCA TGATCTATAG ATCTATAGAT CTCTCGTGGG ATCATTGTTT TTCTCTTGAT TCCCACTTTG TGGTTCTAAG TACTGTGGTT TCCAAATGTG TCAGTTTCAT AGCCTGAAGA ACGAGATCAG CAGCCTCTGT TCCACATACA CTTCATTCTC AGTATTGTTT TGCCAAGTTC TAATTCCATC AGAAGCTGGT CGAGATCCGG AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG TTATTAGGTC CCTCGAAGAG GTTCACTAGG CGCGCCAGGG ACAGCCGCAA CCGGCTTCCC CCACGTCTGG CCAGAGCCAG GACCGCGGCG CTGGGTAGAG CCGCCGCGCT TGCGCCGGGG CAGGGCGGGG AGGGGCAGCG GGGACGCGGC CGGGTGATCC GACCGACCAC GAGCCCGAGG GCGAACGGGT GGGAAGTTGC GGGAAGGTCT GGGGACTGAG CCCGCTCGCG TGGGCCTTGG GGGAGAATCC AGCCGCGTCC CCGGGCCCGA GAGCTGGGAC TCCGGAGCCC CTAAGTTTGA GCGGCCCGGT GGGCGGCGGG GCAAGAGGGG GCGGACGCTG GCCGTCTGAG CCGGCGCGGC CCGGCCCTTC CGGGGCTGCG CGGCTCCCCC GCCTCGGTGC CGGCAAAAAT GTGCCTAGTC ACGGGGCCGC TCTCGGGGGA ACTGAGGTCG CCTTCGGGCT GGGACCCGGA GCCCCTTCGC CGCGCCCCAA GACCTCCTTG AGTGCGGGCT GCGACGCGCT CACCCCGCTG GGCCGTCTGT GGGCGCGGCT TTGCGAAGTC ATCCATCTCT CGGATCACTC TCTGGCAGCC TTGAGCTCTC TTGAAAGCCC AGCCCCGGGA CGAGGGAGGA GCGCCT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001145412, NM_001145413, NM_001313900, NM_001313901, NM_001313902, NM_001313903, NM_001313904, NM_006164
Synonyms	NRF2
Summary	This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

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