PYK2 (PTK2B) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	PYK2
Locus ID	2185
Kit Components	KN203808G1, PYK2 gRNA vector 1 in pCas-Guide CRISPR vector KN203808G2, PYK2 gRNA vector 2 in pCas-Guide CRISPR vector KN203808RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet TTTGTTGGCA GCTGAGGATG GGGGTGGACA GCATCAGTTG ACAGCCTGGC CCTCCACCTG GGCCCTTTCC TGCCTTCTCC ATTCCCATTC CCTCGGGGCC ACTTCTTCTC CAATCTGTTT CTTCTCAGAT TTTCCGCCTT GTCCCTGTTC CTCTCCTGCT ATTTTCTACT GTCTCACTCT TTGCTTTTTC TGCTCAGCTG TGCCTTGGAA CCAAGCTGTG ACCGCCTTCT CTCACAGCTG CCTTTGCTAA GGGTGGGCCC CTTGCCTGGG TGCCAGGGTG GAGGGAGGAA TGGGTCAGAG AGAGGGGCTT CCAGGAGACA GAGCTCATAC CCCTGGGAGC CCCAAGTCTG GGTTTCATAC CTGGATGCTT CTGCAACTTG CCCCTTTCTG CTGCATCTTT CCCAGTCAAG AGAGCCAGGT GCCTTGCAGG GGAGCTTTTG GGAATGGGAG AATATATAGC ATTTGTGTCT GTCTTGGAGC TCGGTGGGTG CTGTCCCTGG GGCCATGAGG TATGTGGGCC TGTGTGGGGC TCTTTCAGGG CTGACCCTCT GCTGTCTCTG CAGGACTGCA ATGTGCCGAT CTTAGCTGCT GCCTGAGAGG TGCAGAGCCC ATGGTGGTGG TACCAGTAGA TGTGGAAAAG GAGGACGTGC GTATCCTCAA GGTCTGCTTC TATAGCAACA GCTTCAATCC TGGGAAAAAC TTCAAACTGG TCAAATGCAC TGTCCAGACG GAGATCCGGG TAAGTGTGAA GTGTCTGCCC TGTCCATCTG TCTGTCCCTC TGTCTTCTTC CTGGGCAGCT GAGCAGCTCT CCTGCAGCCT CGCAGCTCTC CCATATCCAC CCCTGGGTGG AAGAGGTGAG GTGGCATCAG AGAGATGTGC TCAGCCTGCA TCACCAGGCC TGCTCTCCAG ATGTTCCCAT ACTACTTCTC TAGGAGCCTG GGAAACACAT GTTGTAGCCT TTGCTTGAAC CCCAGATAAT TTGTTTGGCC TCTGATTCAA TTCATTTCAA CTTATTAAGT ATTTATGATA CTTGAAGCTC TGCAAGGGAC TTAAAAGCTG CTGTCCTCAA AGAGTTTACA CTCTGATGAA ATAGGAATTA GAGATACACA AGTGATTGCA TTAAAAATAG AACAAAACTG GTATAAGCCT TGTGTGCTGA GCATGTACAG GAAGGCACTG GTGGGGCACT TAGAGGATGA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_004103, NM_173174, NM_173175, NM_173176
Synonyms	CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
Summary	This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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