NGLY1 Human Gene Knockout Kit (CRISPR)

CAT#: KN203447RB

NGLY1 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


NGLY1 Antibody - middle region
    • 100 ul

CNY 5,250.00


Lenti ORF particles, NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2, 200ul, >10^7 TU/mL
    • 200 ul

CNY 12,350.00

Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol NGLY1
Locus ID 55768
Kit Components

KN203447G1, NGLY1 gRNA vector 1 in pCas-Guide CRISPR vector

KN203447G2, NGLY1 gRNA vector 2 in pCas-Guide CRISPR vector

KN203447RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001145293, NM_001145294, NM_001145295, NM_018297
Synonyms CDDG; CDG1V; PNG1; PNGase
Summary This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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