Granulin (GRN) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Granulin
Locus ID	2896
Kit Components	KN202139G1, Granulin gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAAGGCCACCCAGCTCACCA KN202139G2, Granulin gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGATGGTCAGTTCTGCCCTG KN202139-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AGCCCAGACT CCACTGGCCA CCATATTTCC TTTTTCACAA CTTTCTCACC CCTGTGGTTT CCCATGTCAT CATGTGGCCG CTTCCCGCAA GGCCTTAGCG GGGTGCAGGT ATGAACATAG TGTCAGGCAA GGAGGCATCT GGAGGGGAAC CCTGGCTTTT CCTGGGGGGA CTCCCTCCCT GCACCCTAGC CCTGTCCTCT CCCATGGCTA CTGATGCCTT CCCCTCACCC CAGAGGTGGC CCACATCTGC ACAGATCAGA CCCACAAAAA TCACGTCTTC CTGACTCTCA TAAGCCTGCC CAGTGAGGCC CAGGCATTAG GCCATGTGCT GGGGACTCAG ACCCACACAT ATACGCATGT CAGCATTCAT GCTTACAGGT CCGCACATGC TGGGGCAAGT GTCACACACG GGGCGCTGTA GGAAGCTGAC TCTCAGCCCC TGCAGATTTC TGCCTGCCTG GACAGGGAGG TGTTGAGAAG GCTCAGGCAG TCCTGGGCCA GGACCTTGGC CTGGGGCTAG GGTACTGAGT GACCCTAGAA TCAAGGGTGG CGTGGGCTTA AGCAGTTGCC AGACGTTCCT TGGTACTTTG CAGGCAGACC ACCCCGGAGG AGCCAGCTAC AGCTGCTGCC GTCCCCTTCT GGTGAGTGCC CCTCAGCCTA GGCAAGAGCT GGCAGCCTGG GTTTTCCCAA AGGGTCATCT TGGATTGGCC AGAGGAGGAC GCCAGGCACA AGTCTGTGGT TTATCATTTT CCCTGTCTTT CTAGGACAAA TGGCCCACAA CACTGAGCAG GCATCTGGGT GGCCCCTGCC AGGTTGATGC CCACTGCTCT GCCGGCCACT CCTGCATCTT TACCGTCTCA GGGACTTCCA GTTGCTGCCC CTTCCCAGAG GTGAGCGTGC CATCAGCCCA GTGGAGGGGC TTAGGTCTGC ATTTATGCTT TTCCTGCACT CTACCACCTG CAGATAAAAG GGCCCTGCCA ATGCAGGTTT CTCTGTGTTC CACAGGCCGT GGCATGCGGG GATGGCCATC ACTGCTGCCC ACGGGGCTTC CACTGCAGTG CAGACGGGCG ATCCTGCTTC CAAAGATCAG GTGCAGCTGG GGTGTGGGTG CAGGGCAGGC AGACGGGCAG CATGTGGAGT CTGGAACCCA GGAGCCCAGC TGGCGGGGGC AGCCCTGATT CCTGCCCTTG TGCCCTCATT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001012479, NM_002087
Synonyms	acrogranin; GEP; GEP, GP88, PEPI, PGRN, PCDGF; GP88; granulin; granulin-epithelin; PC cell-derived growth factor; PCDGF; PEPI; PGRN; proepithelin; progranulin
Summary	Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]

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