PAM Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	PAM
Locus ID	5066
Kit Components	KN202074G1, PAM gRNA vector 1 in pCas-Guide CRISPR vector KN202074G2, PAM gRNA vector 2 in pCas-Guide CRISPR vector KN202074LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000919, NM_001177306, NM_001319943, NM_138766, NM_138821, NM_138822, NR_033440, NM_001364582, NM_001364584, NM_001364586, NM_001364588, NM_001364589, NM_001364590, NM_001364593, NM_001364583, NM_001364585, NM_001364587, NM_001364591, NM_001364592, NM_001364594, NR_157231
Synonyms	PAL; PHM
Summary	This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

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